Nikon V-1A Fluorescent Microscope Filter Cube for Labophot, Optiphot, Microphot

Product Description

Nikon V-1A fluorescent microscope filter cube. The Nikon number is 78642 and it will fit the Nikon Labophot, Labophot-2, Optiphot, Optiphot-2, Microphot and TMD inverted microscope.

Violet Excitation: V-1A (Longpass Emission)

The Nikon V-1A violet excitation fluorescence filter set is designed with a very narrow excitation passband width of only 10 nanometers to minimize autofluorescence. The longpass barrier filter, with a cut-on wavelength 5 nanometers greater than the dichromatic mirror value, passes all emission wavelengths above 435 nanometers, allowing detection of a wide range of fluorochromes having fluorescence emission in the blue, green, and red spectral regions.

Violet Excitation Filter Block V-1A Specifications

Excitation Filter Wavelengths: 400-410 nanometers (bandpass, 405 CWL)
Dichromatic Mirror Cut-on Wavelength: 430 nanometers (longpass, LP)
Barrier Filter Wavelengths: 435 nanometer cut-on (longpass, LP)

The V-1A fluorescence filter set is designed to perform with a wide range of popular fluorochromes excited with violet wavelengths, and can be considered a general purpose combination for use where autofluorescence must be limited in comparison to that observed with the standard violet filter block. For appropriate fluorophores, the narrow 10-nanometer excitation bandpass (encompassing the mercury arc-discharge lamp h-line at 404 nanometers) significantly reduces autofluorescence, with only a minimal reduction in overall brightness. The V-1A filter combination is specifically recommended when studying the following fluorophores: beta-lactamase (non-ratiometric), Cascade Yellow, catecholamine, CCF2 (GeneBLAzer, formaldehyde-induced fluorescence (FIF), gloxalic acid, hydroxypyrene trisulfonic acid (HPTS), Pacific Blue, pyronine, serotonin, Spectrum Blue, and tetracycline.