Nikon UV-2B Fluorescent Microscope Filter Cube for E400/ 600, TE200/300

Product Description

The Nikon UV-2B filter cube is new item from a former Nikon dealer inventory. It will fit the Nikon Eclipse series of fluorescent microscopes E400, E600, E800, and TE200/300. It is in the catalog with a product number of 96152 with a list price is $1,125.00. The cube is in new condition and still in its' plastic bubble case. The plastic bubble case is not pictured.

Ultraviolet Excitation: UV-2B (Longpass Emission)

The UV-2B combination includes a medium-width bandpass excitation filter (330-380 nanometers) coupled to a longpass barrier filter (420 nanometer cut-on wavelength) to enable collection of signals from a wide spectrum of fluorescent probes. The common dichromatic mirror for the Nikon UV-2 series of filter combinations has a cut-on wavelength of 400 nanometers and reflects greater than 95 percent of the ultraviolet wavelengths between 330 and 390 nanometers. The UV-2B set is similar in spectral profile to the UV-2A, but contains a dichromatic mirror that has a cut-on wavelength shifted to the red (longer wavelengths) by 15 nanometers in order to provide a darker background.

Ultraviolet Excitation Filter Block UV-2B Specifications

Excitation Filter Wavelengths: 330-380 nanometers (bandpass, 355 CWL)
Dichromatic Mirror Cut-on Wavelength: 400 nanometers (longpass, LP)
Barrier Filter Wavelengths: 435 nanometer cut-on (longpass, LP)

The UV-2B fluorescence filter combination is designed to provide all-purpose imaging of fluorescent specimens with ultraviolet excitation, and is similar in filter profiles to the standard set (UV-2A) for this spectral region. The 50-nanometer bandpass excitation filter covers the longer ultraviolet wavelengths and is capable of exciting many of the fluorophores that have marginal absorption in this region. The longpass emission (barrier) filter used in the UV-2Bcombination is designed to collect signals at wavelengths exceeding 435 nanometers, enabling visualization of red and green emission from fluorophores excited in the ultraviolet, but providing a darker background and better contrast than the UV-2A filter combination. The UV-2B filter combination is recommended when studying the following fluorophores: 7-amino-4-methylcoumarin (AMC), and its acetic acid derivatives (AMCA-S and AMCA), aminoquinoline, Aniline Blue, anthroyl stearate, bisaminophenyloxadiazole (BAO 9), bisbenzamide, Calcein Blue, Cascade Blue, dansyl derivatives, 4'-6-diamidino-2-phenylindole (DAPI), dopamine, Fast Blue, FluoroGold, Hoechst 33258/33342, noradrenaline, Nuclear Yellow, pyrene, tetracycline, and True Blue.