Nikon Green G-2B Fluorescent Microscope Filter Cube for E400/600/800, TE200/300

Product Description

This filter fits the Nikon E400/600/800, TE200/300 Inverted series of fluorescent microscopes.

Green Excitation: G-2B (Longpass Emission)

The Nikon G-2B filter set is a variation of the standard combination for use with green excitation wavelengths, and employs a wide (50 nanometers) excitation passband covering essentially the entire green spectral region, from 510 to 560 nanometers. The filter components are similar to those of the G-2A set, except that the dichromatic mirror cut-on wavelength is shifted to longer wavelengths by 10 nanometers (to 575 nanometers), and the emission filter cut-on is shifted 20 nanometers toward the red, to 610 nanometers. The G-2B combination is applicable with a wide range of fluorophores excited by green wavelengths, with emission occurring in the orange through near-infrared regions.

Green Excitation Filter Block G-2B Specifications

Excitation Filter Wavelengths: 510-560 nanometers (bandpass, 535 CWL)
Dichromatic Mirror Cut-on Wavelength: 575 nanometers (longpass, LP)
Barrier Filter Wavelengths: 610 nanometer cut-on (longpass, LP)

The G-2B filter combination is designed to perform as a standard green excitation filter combination, producing bright images from a wide range of fluorochromes, but with darker backgrounds than those obtained with the G-2A set (due to the different dichromatic mirror and emission filter cut-on wavelengths). In addition, the 20-nanometer red shift of the emission filter cut-on excludes more yellow-orange signal, resulting in images that are more reddish visually compared to those from the G-2A filter set. The G-2B set is recommended when studying the following fluorophores: Acridine Red, Alexa Fluors (555, 568, and 594), dichlorodimethoxyfluorescein (JOE), Alizarin Red, BODIPY probes, Calcium Orange, Cy3, Cy3.1.8, dioctadecyl tetramethylindocarbocyanine (DiI), ethidium bromide, FluoroRuby, LDS 751-DNA, MitoTracker Red, R and B-phycoerythrin, POPO-3, PO-PRO-3, propidium iodide (PI), Pyronin B, RedoxSensor Red CC-1, RH probes (237, 414, 421, 795), many rhodamine derivatives, Sevron Brilliant Red, and several SYTO derivatives.