Nikon Green G-2A Fluorescent Microscope Filter Cube for E400/600/800, TE200/300

Product Description

This filter fits the Nikon Eclipse E400/600/800, and TE200/300 Inverted fluorescent microscopes.

Green Excitation: G-2A (Longpass Emission)

The Nikon G-2A filter set is designed as the standard combination for use with green excitation wavelengths, and employs a wide (50-nanometer) excitation passband that covers essentially the entire green spectral region, from 510 to 560 nanometers. Combined with the 590-nanometer cut-on wavelength of the longpass emission filter, the G-2A set enables detection of a wide range of fluorochromes emitting at wavelengths longer than those in the yellow region and extending into the near-infrared spectral zone. The dichromatic mirror has a cut-on wavelength specification of 575 nanometers.

Green Excitation Filter Block G-2A Specifications

Excitation Filter Wavelengths: 510-560 nanometers (bandpass, 535 CWL)
Dichromatic Mirror Cut-on Wavelength: 575 nanometers (longpass, LP)
Barrier Filter Wavelengths: 590 nanometer cut-on (longpass, LP)

The G-2A filter combination is designed to perform with a wide range of fluorochromes excited by green wavelengths, and is considered the standard Nikon filter block for green excitation. The combination of a wide excitation passband (50 nanometers) with a longpass emission (barrier) filter enables detection of fluorescence intensity from a substantial range of fluorophores that emit in the orange through near-infrared spectral regions (restricted to wavelengths longer than the 590-nanometer barrier filter cut-on wavelength). The resulting images are bright, although signal-to-noise ratios are typically lower than those produced with combinations employing bandpass barrier filters (such as the G-2E/C and Cy3). The G-2A set is recommended when studying the following fluorophores: Acridine Red, Alexa Fluors (532, 546, 555, 568, and 594), Alizarin Red, BODIPY probes, Calcium Orange, Cy3, Cy3.1.8, ethidium bromide, FluoroRuby, FM 5-95, hexachlorofluorescein (HEX), LDS 751-DNA, MitoTracker Orange and Red, POPO-3, PO-PRO-3, propidium iodide (PI), Pyronin B, RedoxSensor Red CC-1, RH probes (237, 414, 421, 795), many rhodamine derivatives, Sevron Brilliant Red, SYTO derivatives, SYTOX Orange, and Xylene Orange.