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- FLUORESCENT FILTER CUBES
- Nikon FITC Fluorescent Microscope Filter Cube for 50I, 80I TE2000/3000
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Product Description
The FITC or B-2E/C filter cube is new item from a former Nikon dealer inventory. It will fit the Nikon 50I, 80I and TE2000/3000 Eclipse series of fluorescent Inverted microscopes. It is in the catalog with a product number of 96291 old number and MBE44720 new number. The cube is in new condition and still in its' plastic bubble case. The plastic bubble case is not pictured.
Blue Excitation: B-2E/C (Bandpass Emission)
The Nikon B-2E/C fluorescence filter set is designed as a high-performance combination with a medium-width excitation bandpass (30 nanometers) in order to provide a high signal-to-noise ratio in conjunction with a slightly lowered emission bandpass region compared to the B-1E and B-2E sets. The bandpass emission filter has a center wavelength of 535 nanometers (40-nanometer bandpass), and in similarity to the other filter combinations in this group, the B-2E/C set employs a longpass dichromatic mirror with a cut-on wavelength of 505 nanometers.
Blue Excitation Filter Block B-2E/C Specifications
- Excitation Filter Wavelengths: 465-495 nanometers (bandpass, 480 CWL)
- Dichromatic Mirror Cut-on Wavelength: 505 nanometers (longpass, LP)
- Barrier Filter Wavelengths: 515-555 nanometers (bandpass, 535 CWL)
The B-2E/C filter combination is specifically designed with a 30-nanometer excitation bandpass suitable for imaging specimens labeled with fluorescein isothiocyanate (FITC), and a number of other popular fluorophores used in multiple-labeling fluorescence techniques. The emission bandpass region is somewhat lower in wavelength than that of the B-1E and B-2E filter sets to provide optimum detection of popular fluorochromes such as FITC, BODIPY, Fluo-3, oxacarbocyanine dyes (DiO), and green fluorescent protein (GFP). In addition, the lowered emission filter bandpass results in improved blocking of yellow to red wavelengths, with only green fluorescence appearing in images acquired using the B-2E/Cfilter combination. Among the Nikon blue-excitation filter sets, those in the E-series, in general, produce images with a much higher signal-to-noise ratio and darker background regions than do combinations with longpass emission filters.
The B-2E/C filter set is recommended when investigating the following fluorophores: Acridine Orange bound to DNA (excludes detection of Acridine Orange bound to RNA), Acridine Yellow, Alexa Fluor 488, Auramine O, BODIPY probes, Calcein, Calcium Green, carboxyfluorescein (FAM), coriphosphine O, Cy2, DiO probes, enhanced green and yellow fluorescent proteins (EGFP and EYFP), red-shifted green fluorescent protein (rsGFP), Fluo-3 and similar ratiometric dyes, FITC, LysoTracker derivatives, MitoTracker Green, Oregon Green derivatives, Rhodamine 123 (and similar xanthenes), Spectrum Green, SYTO probes, SYTOX Green, YO-PRO 1, and YOYO 1.
