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- Nikon Cyan BV Fluorescent Microscope Filter Cube for 50I, 80I TE2000/3000
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Product Description
This filter cube is new item from a former Nikon dealer inventory. It will fit the Nikon 50I, 80I and TE2000/3000 Eclipse series of fluorescent Inverted microscopes. It is in the catalog with a product number of 96341. The cube is in new condition and still in its' plastic bubble case. The plastic bubble case is not pictured.
Blue-Violet Excitation: CFP (Bandpass Emission)
The high-performance Nikon CFP (cyan fluorescent protein) filter combination differs from the other three complements in the blue-violet group by employing a bandpass emission filter, whose 40-nanometer passband (460-500 nanometers) restricts detection to fluorochromes emitting in the cyan-blue spectral region. A narrow 20-nanometer excitation passband minimizes autofluorescence and is intended to avoid excitation of certain fluorochromes commonly utilized for dual labeling experiments in conjunction with cyan fluorescent protein. Although primarily designed for imaging of fluorescent proteins, this bandpass emission combination is useful for obtaining images of specimens having multiple labels when one of the fluorophores is efficiently excited in the blue-violet spectral region.
Blue-Violet Excitation Filter Block CFP Specifications
- Excitation Filter Wavelengths: 426-446 nanometers (bandpass, 436 CWL)
- Dichromatic Mirror Cut-on Wavelength: 455 nanometers (longpass, LP)
- Barrier Filter Wavelengths: 460-500 nanometers (bandpass, 480 CWL)
The CFP fluorescence filter set is designed for optimum performance with cyan fluorescent protein excited by blue-violet wavelengths, in particular when utilized for dual labeling techniques in combination with enhanced yellow fluorescent protein. Although originally designed to detect emission from the protein fluorophore, this filter combination is essentially a bandpass emission blue-violet excitation set and can be utilized to examine a wide spectrum of specimens that absorb light in this spectral region.
The excitation bandpass of the CFP combination does not generally excite yellow fluorescent protein (excited at 513 nanometers), and by employing a bandpass emission filter, yellow fluorescence is excluded from detection when the CFP filter set is used. It should be noted that some signal from enhanced green fluorescent protein may be detected with this filter set, and therefore that fluorochrome is not a good candidate for dual labeling with the cyan protein variant. The CFP filter combination is recommended when studying the following fluorophores: ACMA, acridine homodimer, Astrazon Yellow, Atabrine, catecholamine, CFP, Chromomycin A, Genacryl Brilliant Yellow, Genacryl Yellow, Sevron Yellow, SYTO 42 (and 43, 44, 45), and SYTOX Blue.
