Nikon Chroma Texas Red Fluorescent Microscope Filter Labophot, Optiphot, TMD, Microphot

Product Description

This Nikon Chroma filter cube is a demonstrator item from a former Nikon dealer inventory. It will fit the Nikon Labophot, Labophot-2, Optiphot, Optiphot-2, TMD Inverted, and Microphot series of fluorescent microscopes. It is in the catalog with a product number of 41004 The cube is in new condition and still in its' plastic bubble case. The plastic bubble case is not pictured.

Yellow Excitation: Texas Red HYQ (Bandpass Emission)

Ultraviolet, visible, and near-infrared transmission spectral profiles for the Nikon Texas Red HYQ high-performance filter combination. This filter set is one of two in the Nikon yellow excitation group, both of which employ a bandpass emission (barrier) filter instead of a longpass variety, in order to limit interference from fluorophores emitting outside the wavelength region for which the combination is optimized. The 75-nanometer emission window (608 to 683 nanometers) is combined with a medium width (55 nanometers) excitation passband (532 to 587 nanometers) to allow selective excitation and detection of specific popular fluorophores, which are commonly used in multiple labeling experiments. The Texas Red HYQ filter combination is equipped with a 595-nanometer (cut-on wavelength) longpass dichromatic mirror.

Yellow Excitation Filter Block Texas Red HYQ Specifications

Excitation Filter Wavelengths: 532-587 nanometers (bandpass, 560 CWL)
Dichromatic Mirror Cut-on Wavelength: 595 nanometers (longpass, LP)
Barrier Filter Wavelengths: 608-683 nanometers (bandpass, 645 CWL)

The Texas Red HYQ filter combination is designed to increase the brightness of secondary fluorescence without producing a corresponding increase in the signal-to-noise ratio. Steeper passbands are created by increasing the number of interference cavities in both the excitation and emission filters, allowing a closer proximity between the spectral profiles. The resulting wider and steeper filter passbands enable the set to provide more excitation energy and capture a higher level of fluorescence signal. This filter combination is optimized for use with the popular fluorescent probes Texas Red and Cy3.5, when they are used alone or in multiple labeling techniques, and is also recommended for applications with other fluorophores excited by yellow wavelengths. The bandpass emission filter functions to reduce or eliminate interference from fluorophores emitting in the deep red or near-infrared spectral regions. The Texas Red HYQ filter combination is designed as a higher-energy variation of the Nikon Y-2E/C set, with wider excitation and emission filter transmission regions. It is suitable for use with essentially the same group of probes, while offering improved performance for those emitting beyond the upper limits of the Y-2E/C emission band.

Because of increased energy transmission allowed by the expanded excitation and emission passband regions, images produced with the Texas Red HYQ set are typically brighter and appear redder than those from the similar Y-2E/C combination. The Texas Red HYQ filter set is recommended when studying the following fluorophores: Texas Red, CY3.5, aminoactinomycin D (AAD), acid Fuchsin, Alexa Fluor 568 and 594, Alizarin Complexon, BOBO-3, BO-PRO-3, BODIPY, ROX (carboxy-X-rhodamine), DiBAC4(5), HcRed, LDS 751 (bound to RNA), MitoTracker Red, mRFP-1 (monomeric red fluorescent protein), naphthofluorescein, SYPRO Red, SYTO 17 (59,61,64), YO-PRO-3, and YOYO-3.