Nikon Chroma DAPI/ FITC/ TX RED Fluorescent Microscope Filter Labophot Optiphot

Product Description

This filter fits the Nikon Labophot, Labophot-2, Optiphot, Optiphot-2, Microphot, and TMD series of microscopes. It is a Nikon Chroma DAPI/ FITC/ TEXAS RED Triple Band Pass filter cube, product number 61002. The filter is new in its' original plastic container (not pictured). Thanks for looking!

Triple Band Excitation: DAPI-FITC-Texas Red

Ultraviolet and visible transmission spectral profiles for the Nikon DAPI-FITC-Texas Red filter combination filter set is designed for optimal detection of DAPI, FITC (fluorescein isothiocyanate), and Texas Red probes when applied in combination (triple) staining techniques. The DAPI-FITC-Texas Red set incorporates an excitation filter with narrow bandpass windows in the violet (395 to 410 nanometers), blue (490 to 505 nanometers), and green (560 to 580 nanometers) spectral regions.

Three emission (barrier) filter bandpass regions enable detection of blue, green, and red emission from the three fluorochromes simultaneously. Sharp wavelength transitions between transmission and reflection bands for each filter, and application of a dichromatic mirror having multiple bandpass transmission regions chosen to be complementary to the emission and excitation wavelengths, permit triple-band signal detection with minimal crossover (spectral bleed-through), interference, and noise.

Triple Excitation Filter Block DAPI-FITC-Texas Red Specifications

Excitation Filter Wavelengths: 395-410 nanometers (bandpass, 403 CWL), 490-505 nanometers (bandpass, 498 CWL), and 560-580 nanometers (bandpass, 570 CWL)
Dichromatic Mirror Wavelengths: 445-480 nanometers (bandpass), 510-555 nanometers (bandpass), and 590-665 nanometers (bandpass)
Barrier Filter Wavelengths: 450-470 nanometers (bandpass, 460 CWL), 515-545 nanometers (bandpass, 530 CWL), and 600-650 nanometers (bandpass, 625 CWL)

The DAPI-FITC-Texas Red triple band fluorescence filter combination is designed specifically for simultaneous detection of the fluorochromes DAPI, FITC, and Texas Red with minimal crossover between adjacent emission bands, and it can also be employed with other combinations of fluorescent probes that have similar spectral profiles. The short wavelength signal channel of the filter set selects narrowly defined spectral regions for violet excitation and blue emission detection; a second, longer wavelength channel corresponds to blue excitation and green emission detection, and green excitation coupled to red emission constitutes the third band of the filter set. The short wavelength excitation transmission profile is deliberately reduced in size to provide better balance of the blue emission with the other fluorochrome signals, and to minimize photobleaching. This filter set is recommended when studying various combinations of fluorophores chosen from the following three groups: DAPI, blue fluorescent protein (BFP), or Hoechst dyes (violet excitation); FITC, green fluorescent protein (GFP), Cy2, or Alexa Fluor 488 (blue excitation); Texas Red or Alexa Fluor 594 (green excitation).