Nikon Chroma DAPI/ FITC/ TRITC Fluorescent Microscope Filter Labophot Optiphot

Product Description

This filter fits the Nikon Labophot, Labophot-2, Optiphot, Optiphot-2, Microphot, and TMD series of microscopes. It is a Nikon Chroma DAPI/ FITC/ TRITC or Cy3 Triple Band Pass filter cube, product number 61000-V2. The filter is new in its' original plastic container (not pictured). Thanks for looking!

Triple Band Excitation: DAPI-FITC-TRITC

Ultraviolet and visible transmission spectral profiles for the Nikon DAPI-FITC-TRITC filter combination set is designed for optimal detection of DAPI, FITC (fluorescein isothiocyanate), and TRITC (tetramethylrhodamine isothiocyanate) probes when applied in combination, and incorporates an excitation filter with narrow bandpass windows in the violet (385 to 400 nanometers), blue (475 to 490 nanometers), and green (545 to 565 nanometers) spectral regions.

Three emission (barrier) filter bandpass regions allow detection of blue, green, and orange-red emission from up to three fluorochromes simultaneously. Sharp transitions between transmission and reflection bands for each filter, and application of a dichromatic mirror having multiple bandpass transmission regions chosen to be complementary to the emission and excitation wavelengths, permit triple-band signal detection with minimal crossover, interference, and noise.

Triple Excitation Filter Block DAPI-FITC-TRITC Specifications

Excitation Filter Wavelengths: 385-400 nanometers (bandpass, 393 CWL), 475-490 nanometers (bandpass, 483 CWL), and 545-565 nanometers (bandpass, 555 CWL)
Dichromatic Mirror Wavelengths: 435-470 nanometers (bandpass), 500-540 nanometers (bandpass), and 570-645 nanometers (bandpass)
Barrier Filter Wavelengths: 450-465 nanometers (bandpass, 458 CWL), 505-535 nanometers (bandpass, 520 CWL), and 580-620 nanometers (bandpass, 600 CWL)

The DAPI-FITC-TRITC triple band fluorescence filter combination is designed specifically for simultaneous detection of the fluorochromes DAPI, FITC, and TRITC with minimal crossover (spectral bleed-through) between adjacent bands, and it can also be employed with other combinations of fluorescent probes that have similar spectral profiles. The short wavelength signal channel of the filter set selects narrowly defined spectral regions for violet excitation and blue emission detection; a second, longer-wavelength channel corresponds to blue excitation and green emission detection, and green excitation coupled to orange-red emission constitutes the third band of the filter set. The short wavelength excitation transmission is deliberately reduced in size to provide better balance of the blue emission with the other fluorochrome signals, and also to minimize photobleaching. This filter set is recommended when studying various combinations of fluorophores chosen from the following three groups: DAPI, blue fluorescent protein (BFP), or Hoechst dyes (violet excitation); FITC, green fluorescent protein (GFP), Cy2, or Alexa Fluor 488 (blue excitation); TRITC, DsRed, Cy3, Alexa Fluor 546, or Mito Tracker Red CMXRos (green excitation).