Nikon BV-1A Fluorescent Microscope Filter Cube for Labophot, Optiphot, Microphot

Product Description

Pictured is a Nikon fluorescent filter cube BV-1A designed to fit the following microscopes: Nikon labophot, labophot-2, optiphot, opotiphot-2, microphot, and TMD inverted. It is in new condition having been used as a dealer demonstrator. The Nikon product number is 78643 and carried a llast list price of $1,023.00. Thank you for looking!

Blue-Violet Excitation: BV-1A (Longpass Emission)

Ultraviolet, visible, and near-infrared transmission spectral profiles for the Nikon BV-1A filter combination. This set is designed with a very narrow excitation bandpass width of 10 nanometers to minimize autofluorescence. The longpass barrier filter, with cut-on wavelength that is 15 nanometers greater than the dichromatic mirror value, passes all fluorescence wavelengths above 470 nanometers, allowing detection of a broad range of fluorochromes emitting in the blue, green, yellow, orange, and red regions of the visible spectrum.

Blue-Violet Excitation Filter Block BV-1A Specifications

Excitation Filter Wavelengths: 430-440 nanometers (bandpass, 435 CWL)
Dichromatic Mirror Cut-on Wavelength: 455 nanometers (longpass, LP)
Barrier Filter Wavelengths: 470 nanometer cut-on (longpass, LP)

The BV-1A fluorescence filter set is designed to perform with a wide range of fluorochromes excited by blue-violet wavelengths, and can be considered a general purpose combination for use where autofluorescence must be limited in comparison to that observed with the standard blue-violet filter block. This filter combination is particularly suited for applications with mercury arc-discharge lamps, which have a prominent spectral line (known as the g-line) at 436 nanometers. For investigations involving living or fixed cells and tissues, the narrow 10-nanometer excitation passband significantly reduces autofluorescence. The BV-1A filter combination is recommended when studying the following fluorophores: aminochloromethoxyacridine (ACMA), acriflavin, Alexa Fluor 430, Astrazon Yellow, atabrine, Calcofluor White, Chromomycin A, chlorotetracycline (CTC), Lucifer Yellow derivatives, quinacrine mustard, and thioflavine S.