Nikon Blue Violet BV-2B Fluorescent Microscope Filter Cube for E400/ 600/800, TE200/300

Product Description

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This filter fits the Nikon Eclipse E400/600/800, TE200/300 series of microscopes.

Blue-Violet Excitation: BV-2B (Longpass Emission)

This filter set is a variation of the BV-2A block, and employs similar components except that each is red-shifted (to a longer wavelength) by 5 nanometers in the center (bandpass) or cut-on (longpass) wavelength. The modified combination results in images with darker backgrounds and higher contrast than those produced with the BV-2A set. The filter combination in the Nikon BV-2B set consists of a medium-width bandpass excitation filter (405-445 nanometers) coupled with a longpass barrier filter (475-nanometer cut-on wavelength) to enable signal collection from a wide range of fluorescent probes. The BV-2B filter set also utilizes a dichromatic mirror with a 460-nanometer cut-on wavelength specification, providing a 15-nanometer separation from the barrier filter cut-on.

Blue-Violet Excitation Filter Block BV-2B Specifications

Excitation Filter Wavelengths: 405-445 nanometers (bandpass, 425 CWL)
Dichromatic Mirror Cut-on Wavelength: 460 nanometers (longpass, LP)
Barrier Filter Wavelengths: 475 nanometer cut-on (longpass, LP)

The BV-2B fluorescence filter set is designed to perform with a wide range of fluorochromes excited by blue-violet wavelengths, and is a minor variation of the standard Nikon blue-violet filter block (the BV-2A). By employing a medium-width excitation bandpass in conjunction with a longpass barrier filter, this filter set (along with the BV-2A set) produces the brightest images of the blue-violet group. The BV-2B filter combination is recommended when studying the following fluorophores: aminochloromethoxyacridine (ACMA), acridine homodimer, acriflavin, aminopyrene trisulfate (APTS), Alexa Fluor 430, Astrazon Yellow, atabrine, berberine sulfate, beta-lactamase (non-ratiometric), catecholamine, cyan fluorescent protein (CFP), Calcofluor White, Chromomycin A, chlorotetracycline (CTC), Lucifer Yellow, mithramycin, quinacrine mustard, Sevron Yellow, and thioflavine S.