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- Nikon B-3A Fluorescent Microscope Filter Cube for Labophot, Optiphot, TMD
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Nikon B-3A Fluorescent Microscope Filter Cube for Labophot, Optiphot, TMD
Product Description
The Nikon B-3A filter cube is new item from a former Nikon dealer inventory. It will fit the Nikon Labophot, Labophot-2, Optiphot, Optiphot-2, TMD and Microphot series of fluorescent microscopes. It is in the catalog with a product number of 78659 with a list price is $1,032.00. The cube is in new condition and still in its' plastic bubble case. The plastic bubble case is not pictured.
Blue Excitation: B-3A (Longpass Emission)
The Nikon B-3A fluorescence filter set is equipped with a very broad excitation passband range (70 nanometers), providing a much wider absorption window than any of the other combinations in the blue-excitation series. The wide excitation band enables the filter set to be employed with tungsten-halogen illumination, although it is also useful with arc-discharge lamps when investigating probes having very weak signals. The longpass barrier (emission) filter has a cut-on wavelength of 520 nanometers and is capable of transmitting a significant amount of the signal from green, yellow, orange, and red fluorophores that have absorption bands in the blue wavelength region. Similar to the other filter combinations in this class, the B-3A has a longpass dichromatic mirror with a cut-on wavelength of 510 nanometers.
Blue Excitation Filter Block B-3A Specifications
- Excitation Filter Wavelengths: 420-490 nanometers (bandpass, 455 CWL)
- Dichromatic Mirror Cut-on Wavelength: 510 nanometers (longpass, LP)
- Barrier Filter Wavelengths: 520 nanometer cut-on (longpass, LP)
The longpass emission filter contained in the B-3A combination transmits significantly more signal than any of the E-series filter sets, and is comparable to the other longpass (B-1A and B-2A) combinations in the blue-excitation class (with the exception of displaying a much higher noise level). The set is designed to enable the use of tungsten-halogen illumination for fluorochromes excited in the blue spectral region and employs a dichromatic mirror optimized for transmitting fluorescence emission in the green, yellow, orange, and red regions. The 510-nanometer dichromatic mirror cut-on wavelength coupled with the longpass barrier filter (cut-on wavelength of 520 nanometers) is similar to the other blue-excitation filter combinations.
Images produced by the B-3A filter set are very bright (especially with arc-discharge illumination), but have a significantly lower signal-to-noise ratio than images obtained utilizing bandpass emission filters. As a result, the background regions are lighter than those from bandpass emission filters, or in some cases, from other longpass filter combinations (depending upon the illumination source and other factors). Fluorescence emission in the green, yellow, orange, and red regions is often visible in images collected using the B-3Afilter combination. This set is recommended when investigating the following fluorophores: Acridine Orange, Acridine Red, Alexa Fluor 430, Alexa Fluor 488, chlorophyll (autofluorescence), fluorescein probes, LysoTracker derivatives, phosphine, quinacrine mustard, Sevron probes, and tetracycline. In general, images generated with the B-3A filter combination exhibit far more background signal than is observed with the other blue excitation sets.
The Nikon B-3A filter was designed to be used with a tungsten-halogen illumination system. The Nikon number was 78659 and was priced at $822 when it was last offered. These filters are hard to find and even more hard to find in this condition.
