Nikon B-2E Fluorescent Microscope Filter Cube E400/ 600, TE200/300

Product Description

This B-2E filter cube is new item from a former Nikon dealer inventory. It will fit the Nikon Eclipse series of fluorescent microscopes, such as E400/600/800 and TE200/300 Inverted microscope. It is in the catalog with a product number of 96155 or MBE342AP. The cube is in new condition and still in its' plastic bubble case. The plastic bubble case is not pictured.

Blue Excitation: B-2E (Bandpass Emission)

Ultraviolet, visible, and near-infrared transmission spectra. This filter set is designed with a wide excitation bandpass (40 nanometers) in order to provide a relatively high signal-to-noise ratio with an adequate spectral absorption and excitation profile for most fluorophores responding to blue wavelengths. The bandpass emission filter has a center wavelength of 540 nanometers (40-nanometer bandpass), and in similarity to the other filter combinations in this group, the B-2Eset employs a longpass dichromatic mirror, which has a cut-on wavelength of 505 nanometers.

Blue Excitation Filter Block B-2E Specifications

Excitation Filter Wavelengths: 450-490 nanometers (bandpass, 470 CWL)
Dichromatic Mirror Cut-on Wavelength: 505 nanometers (longpass, LP)
Barrier Filter Wavelengths: 520-560 nanometers (bandpass, 540 CWL)

The B-2E filter combination is specifically designed with a wide excitation passband suitable for imaging specimens labeled with fluorescein isothiocyanate (FITC) and derivatives, in addition to providing sufficient excitation energy to enhance emission (relative to the B-1Eset) from a range of fluorochromes with similar absorption characteristics. In order to exclude crossover from yellow, orange, and red wavelengths, the filter set incorporates a bandpass barrier filter that is identical to that included in the B-1E set, but with a longer bandpass center wavelength than that of the B-2E/C combination. Among the Nikon blue excitation filter sets, those in the E series, in general, produce images with a much higher signal-to-noise ratio than do combinations with longpass emission filters (the A filter category). In addition, the image background regions appear significantly darker with the bandpass filter combinations. Fluorescence emission occurring at longer wavelengths than green (the yellow, orange, and red spectral regions) is excluded from images collected using the B-2E filter combination.

The B-2E filter set is recommended when investigating the following fluorophores: Acridine Orange-DNA (excludes detection of Acridine Orange-RNA), Acridine Yellow, Alexa Fluor 488, BODIPY probes, Calcein, Calcium Green, carboxyfluorescein (FAM), Cy2, DiO probes, enhanced green and yellow fluorescent proteins (EGFP and EYFP), red-shifted green fluorescent protein (RsGFP), FITC, LysoTracker derivatives, MitoTracker Green, Oregon Green, Rhodamine 123 (and related xanthenes), Spectrum Green, SYTO probes, SYTOX Green, YO-PRO 1, and YOYO 1.