Nikon B-2A Fluorescent Microscope Filter Cube for Labophot, Optiphot, Microphot

Product Description

Pictured is a Nikon fluorescent filter cube BV-2A designed to fit the following microscopes: Nikon labophot, labophot-2, optiphot, opotiphot-2, microphot, and TMD inverted. It is in new condition having been used as a dealer demonstrator. The Nikon product number is 78648 and carried a llast list price of $1,023.00. Thank you for looking!

The cube is new and still in its' plastic bubble case. The plastic bubble case is not pictured.

The Nikon B-2A fluorescence filter set is designed to provide an expanded absorption window for fluorophores, when compared to the B-1A combination, by utilizing a medium sized bandpass excitation filter (40 nanometers) rather than a narrow excitation passband. The longpass barrier (emission) filter has a cut-on wavelength of 515 nanometers and is capable of transmitting a significant amount of the signal from green, yellow, and red fluorophores that have absorption bands in the central blue wavelength region. In addition, the B-2A combination employs a longpass dichromatic mirror with a cut-on wavelength of 500 nanometers, 5 nanometers below that of other filter sets in the blue-excitation series.

lue Excitation Filter Block B-2A Specifications

Excitation Filter Wavelengths: 450-490 nanometers (bandpass, 470 CWL)
Dichromatic Mirror Cut-on Wavelength: 510 nanometers (longpass, LP)
Barrier Filter Wavelengths: 510 nanometer cut-on (longpass, LP)

Images produced by the B-2A filter combination are brighter, but have a lower signal-to-noise ratio than their bandpass counterparts, typically resulting in lighter background areas compared to images acquired with the bandpass emission filter sets. Fluorescence emission in the yellow, orange, and red regions is often visible in images collected using the B-2A filter combination. This set is recommended when investigating the following fluorophores: fluorescein isothiocyanate (FITC), Acridine Orange, Acridine Yellow, Alexa Fluor 488, Auramine O, BOBO-1, BO-PRO-1, Calcein, Coriphosphine O, BODIPY FL, Fluo-3, green fluorescent protein (GFP; and red-shifted variants), oxacarbocyanine dyes (such as DiO), SYTO, and SYTOX Green.